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Chem Impex International
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AG Scientific
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ViroPharma inc
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Savara Pharmaceuticals
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Chemwerth Inc
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Noridem Enterprises Ltd
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MultiCell Technologies
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Chemie GmbH
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TOKU-E
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Image Search Results
Journal: ACS Omega
Article Title: Electrospun Chitosan-Based Nanofibrous Coating for the Local and Sustained Release of Vancomycin
doi: 10.1021/acsomega.3c08113
Figure Lengend Snippet: Microscopic characterization of nanofibrous mat SEM images of formulations of vancomycin nanofibers at magnifications of 5000x and corresponding size distribution histograms. (A) Formulation with chitosan in HFIP. (B) Formulation with chitosan and PVA in acetic acid solution (F2). (C) Formulation with chitosan and PEO in acetic acid solution (F4). Chitosan alone proved to be extremely difficult to spin due to its limited solubility (A), and for this reason, the addition of a copolymer such as PVA and PEO was explored to enhance the electrospinnability properties of chitosan (B,C).
Article Snippet:
Techniques: Formulation, Solubility
Journal: Frontiers in Microbiology
Article Title: Glycopeptide Antibiotic Teicoplanin Inhibits Cell Entry of SARS-CoV-2 by Suppressing the Proteolytic Activity of Cathepsin L
doi: 10.3389/fmicb.2022.884034
Figure Lengend Snippet: Teicoplanin specifically inhibited the entry of pseudotyped SARS-CoV-2. (A) Schematic of the pseudotyped virus entry upon drug treatment assay. To package different pseudotyped viruses, psPAX2 plasmids, and pHIV-Luciferase plasmids were co-transfected into HEK293T cells with pcDNA3.1-SARS-CoV S, pcDNA3.1-SARS-CoV-2 S, and pCMV-VSV-G plasmids, respectively. HEK293T-hACE2 high cells were incubated with drugs and different pseudotyped viruses. The amounts of luciferase within cells were measured 48 h post infection. (B) Chemical structure of teicoplanin. (C) HEK293T-hACE2 high cells were treated with 0, 25, and 50 μM teicoplanin, respectively, followed by infecting with different pseudotyped viruses including SARS-CoV S/HIV-1, SARS-CoV-2 S/HIV-1, and VSV-G/HIV-1. The intracellular luciferase activity was measured 48 h post infection ( n = 3). (D) Chemical structure of dalbavancin. (E) HEK293T-hACE2 high cells were treated as in (C) , except that the drug was replaced with dalbavancin ( n = 3). (F) Chemical structure of vancomycin. (G) HEK293T-hACE2 high cells were treated as in (C) , except that the drug was replaced with vancomycin ( n = 3). Data in (C,E,G) represented as mean ± SEM in triplicate. P -values were calculated by two-way ANOVA with Dunnett’s multiple comparisons test which compared the mean of each group with the mean of the control group. ns p ≥ 0.05, *** p < 0.001.
Article Snippet: For virus infection in drug treatment experiment, HEK293T-hACE2 high cells were incubated with serially diluted drugs including teicoplanin (Selleck, S1399, United States), dalbavancin (Selleck, S4848, United States),
Techniques: Virus, Luciferase, Transfection, Incubation, Infection, Activity Assay, Control